| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Smith, Lyla E (12)
|
MDCAC |
30.25 |
30.17 |
-0.08 |
| 2 |
Handwerger, Jenna E (11)
|
MDEST |
34.33 |
32.77 |
-1.56 |
| 3 |
Stover, Mina K (12)
|
MDEST |
36.24 |
33.96 |
-2.28 |
| 4 |
Kelley, Fiona M (12)
|
MDSPRC |
38.00 |
34.23 |
-3.77 |
| 5 |
Klaus, Leah M (11)
|
MDEST |
33.21 |
34.51 |
1.30 |
| 6 |
Demmin, Emma (11)
|
MDEST |
36.78 |
35.51 |
-1.27 |
| 7 |
Birdsall, Caroline R (12)
|
MDEST |
38.85 |
36.49 |
-2.36 |
| 8 |
Uhlmann, Alexandra R (11)
|
MDCAC |
37.55 |
36.69 |
-0.86 |
| 9 |
Cray, Aliceanna J (12)
|
MDJCC |
42.73 |
38.50 |
-4.23 |
| 10 |
Mester, Teresa A (11)
|
MDEST |
38.39 |
38.60 |
0.21 |
|
Stover, Madison K (12)
|
MDEST |
34.13 |
DQ |
- |